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cd14 microbeads kit  (Miltenyi Biotec)


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    Miltenyi Biotec cd14 microbeads kit
    Cd14 Microbeads Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 4711 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd14+microbead+kit/CD14+MicroBeads%2C+human/pm42843813-37-6-9
    Average 98 stars, based on 4711 article reviews
    cd14 microbeads kit - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    other:

    Article Title: The human cytomegalovirus-encoded pUS28 antagonizes CD4+ T cell recognition by targeting CIITA
    Article Snippet: UoC- B6 (CVCL_A304) cells were grown in RPMI 1640 medium (Gibco) supplemented with 10% (v/v) FCS (Sigma- Aldrich) and 100 μg/ml streptomycin/100 U/ml penicillin (Gibco) at 37 °C in 5% CO2.

    Isolation:

    Article Title: The human cytomegalovirus-encoded pUS28 antagonizes CD4+ T cell recognition by targeting CIITA
    Article Snippet: .. To obtain human dendritic cells, monocytes were isolated from PBMCs using a CD14 MicroBead kit (Miltenyi) according to the manufacturer’s instructions, and purity was validated by flow cytometry. .. For maturation of monocytes into dendritic cells, monocytes were first cultured for 3 d in RPMI 1640 (c.c.pro) supplemented with 10% (v/v) heat-inactivated human serum (Merck), 50 U/ml IL-2 (Miltenyi), 10 ng/ml IL-7, 5 ng/ml IL-15 (R&D Systems), 10 ng/ml IL-4 (R&D Systems) and 100 ng/ml GMCSF (Miltenyi).

    Article Title: Compounds and methods targeting epiregulin
    Article Snippet: Briefly, PBMCs are isolated using density-gradient centrifugation with Ficoll (#17-1440-02, GE Healthcare) and Sepmate 50 (#15450, STEMCELL Technologies) from LRS-WBC. .. CD14+ monocytes are isolated using positive selection with a CD14+ microbead kit (#130-050-201, Miltenyi Biotec) following the manufacturer's manual. .. Cells are then cultured at 1 million/ml with 1000 unit/ml GM-CSF and 600 unit/ml IL-4 for 6 days to drive to immature dendritic cells (MDDC) in RPMI medium with L-glutamine and 25 mM HEPES supplemented with 10% FBS, 1 mM sodium pyruvate, 1× penicillin-streptomycin, 1× non-essential amino acids, and 55 μM 2-mercaptoethanol (hereafter referred to complete RPMI medium or medium, purchased from Life Technologies).

    Article Title: Monocyte-macrophage membrane expression of IL-1R2 is a severity biomarker in sepsis
    Article Snippet: Data were analyzed with FlowJo software (Treestar, Ashland, US). .. Monocytes were isolated from buffy coats from healthy donors using the CD14 MicroBead Kit (Miltenyi Biotec). .. The cells were primed for 4 days with 50 ng/mL of recombinant human (rh) Macrophage Colony-Stimulating Factor (M-CSF) or rh Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) (Peprotech) in RPMI 1640 (Lonza) with 10% FBS (Lonza), 1% l -glutamine (Lonza) and 1% Penicillin-Streptomycin (Lonza).

    Article Title: Monocyte-macrophage membrane expression of IL-1R2 is a severity biomarker in sepsis.
    Article Snippet: Data were analyzed with FlowJo software (Treestar, Ashland, US). .. Monocytes were isolated from buffy coats from healthy donors using the CD14 MicroBead Kit (Miltenyi Biotec). .. The cells were primed for 4 days with 50 ng/mL of recombinant human (rh) Macrophage ColonyStimulating Factor (M-CSF) or rh Granulocyte-Macrophage ColonyStimulating Factor (GM-CSF) (Peprotech) in RPMI 1640 (Lonza) with 10% FBS (Lonza), 1% L-glutamine (Lonza) and 1% Penicillin-Streptomycin (Lonza).

    Article Title: Circulating pre-osteoclasts are primed for osteoclast fate and synovial tissue homing in psoriatic arthritis
    Article Snippet: .. After PBMC isolation as described above, monocyte enrichment was carried out using positive selection with the CD14 MicroBead kit (Miltenyi, 130-050-201) as per the manufacturer’s instructions. .. Cells were blocked with FACS buffer at 80μL per 10 million cells, and labelled with CD14 MicroBeads at 20μL per 10 million cells for 15minutes at 4°C.

    Flow Cytometry:

    Article Title: The human cytomegalovirus-encoded pUS28 antagonizes CD4+ T cell recognition by targeting CIITA
    Article Snippet: .. To obtain human dendritic cells, monocytes were isolated from PBMCs using a CD14 MicroBead kit (Miltenyi) according to the manufacturer’s instructions, and purity was validated by flow cytometry. .. For maturation of monocytes into dendritic cells, monocytes were first cultured for 3 d in RPMI 1640 (c.c.pro) supplemented with 10% (v/v) heat-inactivated human serum (Merck), 50 U/ml IL-2 (Miltenyi), 10 ng/ml IL-7, 5 ng/ml IL-15 (R&D Systems), 10 ng/ml IL-4 (R&D Systems) and 100 ng/ml GMCSF (Miltenyi).

    Selection:

    Article Title: Compounds and methods targeting epiregulin
    Article Snippet: Briefly, PBMCs are isolated using density-gradient centrifugation with Ficoll (#17-1440-02, GE Healthcare) and Sepmate 50 (#15450, STEMCELL Technologies) from LRS-WBC. .. CD14+ monocytes are isolated using positive selection with a CD14+ microbead kit (#130-050-201, Miltenyi Biotec) following the manufacturer's manual. .. Cells are then cultured at 1 million/ml with 1000 unit/ml GM-CSF and 600 unit/ml IL-4 for 6 days to drive to immature dendritic cells (MDDC) in RPMI medium with L-glutamine and 25 mM HEPES supplemented with 10% FBS, 1 mM sodium pyruvate, 1× penicillin-streptomycin, 1× non-essential amino acids, and 55 μM 2-mercaptoethanol (hereafter referred to complete RPMI medium or medium, purchased from Life Technologies).

    Article Title: Circulating pre-osteoclasts are primed for osteoclast fate and synovial tissue homing in psoriatic arthritis
    Article Snippet: .. After PBMC isolation as described above, monocyte enrichment was carried out using positive selection with the CD14 MicroBead kit (Miltenyi, 130-050-201) as per the manufacturer’s instructions. .. Cells were blocked with FACS buffer at 80μL per 10 million cells, and labelled with CD14 MicroBeads at 20μL per 10 million cells for 15minutes at 4°C.



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    Representative Identification of Isolated Human Monocytes and Imaging Setup for Real-Time Ca 2+ Measurements (A) Representative fluorescence images of isolated human monocytes immunolabeled with <t>anti-CD14.</t> Nuclei were stained with DAPI to visualize cell number and morphology. CD14 + cells are shown in the corresponding fluorescence channel, and the merged panel illustrates the colocalization of DAPI and CD14 signals, confirming the identity and purity of the isolated monocyte population. (B) Illustration of the experimental configuration used for Ca 2+ imaging. Monocytes were plated on poly-L-lysine–coated coverslips and incubated at 37 °C in a humidified 5% CO 2 atmosphere before dye loading (a). During imaging, cells were superfused with physiological saline solution while Fura-2 fluorescence was recorded using a direct fluorescence microscope equipped with a perfusion chamber. The schematic depicts the chamber for 13 mm and 35 mm coverslips respectively (c, e), perfusion inlet/outlet (d), Fura-2 loading in physiological buffer, and the microscope setup used to acquire real-time Ca 2+ signals.
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    Representative Identification of Isolated Human Monocytes and Imaging Setup for Real-Time Ca 2+ Measurements (A) Representative fluorescence images of isolated human monocytes immunolabeled with <t>anti-CD14.</t> Nuclei were stained with DAPI to visualize cell number and morphology. CD14 + cells are shown in the corresponding fluorescence channel, and the merged panel illustrates the colocalization of DAPI and CD14 signals, confirming the identity and purity of the isolated monocyte population. (B) Illustration of the experimental configuration used for Ca 2+ imaging. Monocytes were plated on poly-L-lysine–coated coverslips and incubated at 37 °C in a humidified 5% CO 2 atmosphere before dye loading (a). During imaging, cells were superfused with physiological saline solution while Fura-2 fluorescence was recorded using a direct fluorescence microscope equipped with a perfusion chamber. The schematic depicts the chamber for 13 mm and 35 mm coverslips respectively (c, e), perfusion inlet/outlet (d), Fura-2 loading in physiological buffer, and the microscope setup used to acquire real-time Ca 2+ signals.
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    Image Search Results


    Representative Identification of Isolated Human Monocytes and Imaging Setup for Real-Time Ca 2+ Measurements (A) Representative fluorescence images of isolated human monocytes immunolabeled with anti-CD14. Nuclei were stained with DAPI to visualize cell number and morphology. CD14 + cells are shown in the corresponding fluorescence channel, and the merged panel illustrates the colocalization of DAPI and CD14 signals, confirming the identity and purity of the isolated monocyte population. (B) Illustration of the experimental configuration used for Ca 2+ imaging. Monocytes were plated on poly-L-lysine–coated coverslips and incubated at 37 °C in a humidified 5% CO 2 atmosphere before dye loading (a). During imaging, cells were superfused with physiological saline solution while Fura-2 fluorescence was recorded using a direct fluorescence microscope equipped with a perfusion chamber. The schematic depicts the chamber for 13 mm and 35 mm coverslips respectively (c, e), perfusion inlet/outlet (d), Fura-2 loading in physiological buffer, and the microscope setup used to acquire real-time Ca 2+ signals.

    Journal: STAR Protocols

    Article Title: Protocol for isolating and culturing human monocytes from peripheral blood and measuring intracellular calcium dynamics using Fura-2

    doi: 10.1016/j.xpro.2026.104511

    Figure Lengend Snippet: Representative Identification of Isolated Human Monocytes and Imaging Setup for Real-Time Ca 2+ Measurements (A) Representative fluorescence images of isolated human monocytes immunolabeled with anti-CD14. Nuclei were stained with DAPI to visualize cell number and morphology. CD14 + cells are shown in the corresponding fluorescence channel, and the merged panel illustrates the colocalization of DAPI and CD14 signals, confirming the identity and purity of the isolated monocyte population. (B) Illustration of the experimental configuration used for Ca 2+ imaging. Monocytes were plated on poly-L-lysine–coated coverslips and incubated at 37 °C in a humidified 5% CO 2 atmosphere before dye loading (a). During imaging, cells were superfused with physiological saline solution while Fura-2 fluorescence was recorded using a direct fluorescence microscope equipped with a perfusion chamber. The schematic depicts the chamber for 13 mm and 35 mm coverslips respectively (c, e), perfusion inlet/outlet (d), Fura-2 loading in physiological buffer, and the microscope setup used to acquire real-time Ca 2+ signals.

    Article Snippet: CD14 MicroBeads isolation kit , Miltenyi Biotec , Cat# 130-050-201.

    Techniques: Isolation, Imaging, Fluorescence, Immunolabeling, Staining, Incubation, Saline, Microscopy